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尹恒 出生年月：1982.10 工作单位：中国科学院大连化学物理研究所 职称/职务：研究员/研究组组长 地址：大连化物所1805组，辽宁省大连市中山路457号，116023 电话：0411-84379061； |
个人简介
1982年10月生，2003年毕业于大连理工大学精细化工专业获学士学位；2008年毕业于中科院大连化物所生物化工专业获博士学位，期间于2004-2006年在复旦大学糖复合物卫生部重点实验室进行联合培养。毕业后留所工作至今，其间于2010-2011年在丹麦奥胡斯大学做访问学者。2011年任副研究员，2015年任研究员。现任天然产物及糖工程研究组组长，辽宁省碳水化合物研究重点实验室主任，大连市糖类农用制剂工程研究中心主任。
研究方向主要为糖工程及酶工程、糖生物学及植物免疫。在糖工程领域，致力于将天然碳水化合物资源转化为功能产品或能源产品，重点研究如何绿色高效的从多糖降解制备得到寡糖，并对此过程中重要的生物酶进行创制及机制研究。开发得到海藻酸钠裂解酶、葡聚糖酶等多种新型多糖降解酶，这些酶除应用于功能寡糖生产外，已与知名酶制剂公司合作开发产品，进入市场。通过酶法制备得到海藻酸钠寡糖等系列寡糖，通过和企业合作，开发生物农药、肥料多种获农药登记证等国家相关证书，在全国多地多种作物上实现了寡糖农用制剂大规模推广应用，产生较大社会与经济效益；相关产品已在东南亚一带一路国家及美国投入应用。在糖生物学领域，主要研究植物中糖基化修饰等基础糖生物学问题，解析植物体内重要单糖的合成途径并明确途径中关键酶的功能；研究外源寡糖在植物上调节植物免疫作用的功能、机制与应用，系统研究了寡糖激活植物免疫诱导植物抗病的作用机制。
累计主持国家重点研发计划、国家自然科学基金、国家科技支撑计划、中科院STS等10余项课题。主编外文专著一部，参与编著中文著作三部，发表SCI论文60余篇，申请专利50余项。现为中科院青年创新促进会会员、全国农药标委会生物农药分委会委员、中国生物工程学会糖生物工程专委会委员、中国生化与分子生物学会糖复合物专委会委员。获得海洋科学技术奖一等奖、辽宁省科技进步二等奖、张树政糖科学优秀青年奖、大连市杰出青年科技人才、兴辽英才等科技奖励。
研究工作经历
2015/07-，中科院大连化物所，研究员、研究组组长;
2015/05-2015/07，中科院大连化物所，副研究员、研究组组长;
2013/05-2015/05，中科院大连化物所，副研究员、研究组召集人;
2012/01-2013/05，中科院大连化学物理研究所生物技术部，副研究员、研究组组副组长
2011/10-2012/01，中国科学院大连化学物理研究所，生物技术部，副研究员
2008/09-2011/10，中国科学院大连化学物理研究所，生物技术部，助研，其中：
2010/03-2011/02，丹麦奥胡斯大学，食品科学系，公派访问学者
受教育经历
2003/09-2008/08，中国科学院大连化学物理研究所，生物化工，硕博连读，其中：
2003/09-2004/09, 中国科学院研究生院基础课学习
2004/12-2006/08, 复旦大学上海医学院基因中心联合培养
1999/09-2003/06，大连理工大学，化工学院，学士
主要论著
期刊论文
1. Z. Yu, B. Zhu, W. Wang, H. Tan, and H. Yin, Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp. International Journal of Biological Macromolecules 112 (2018) 937-942.
2. J. Zheng, G. Cheng, Q. Li, S. Jiao, C. Feng, X. Zhao, H. Yin, Y. Du, and H. Liu, Chitin Oligosaccharide Modulates Gut Microbiota and Attenuates High-Fat-Diet-Induced Metabolic Syndrome in Mice. Marine Drugs 16 (2018) 1-17.
3.J. Zheng, S. Jiao, Q. Li, P. Jia, H. Yin, X. Zhao, Y. Du, and H. Liu, Antrodia cinnamomea Oligosaccharides Suppress Lipopolysaccharide-Induced Inflammation through Promoting O-GlcNAcylation and Repressing p38/Akt Phosphorylation. Molecules 23 (2018) 1-16.
4.D. Liu, S. Jiao, G. Cheng, X. Li, Z. Pei, Y. Pei, H. Yin, and Y. Du, Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell. International Journal of Biological Macromolecules 111 (2018) 1083-1090.
5. J. Zheng, X. Yuan, G. Cheng, S. Jiao, C. Feng, X. Zhao, H. Yin, Y. Du, and H. Liu, Chitosan oligosaccharides improve the disturbance in glucose metabolism and reverse the dysbiosis of gut microbiota in diabetic mice. Carbohydrate Polymers 190 (2018) 77-86.
6.B. Zhu, F. Ni, Y. Sun, X. Zhu, H. Yin, Z. Yao, and Y. Du, Insight into carrageenases: major review of sources, category, property, purification method, structure, and applications. Critical Reviews in Biotechnology (2018) 1-16.
7. C. Badiali, G. De Angelis, G. Simonetti, E. Brasili, E.D.C. Tobaruela, E. Purgatto, H. Yin, A. Valletta, and G. Pasqua, Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts. Plant Cell Reports 37 (2018) 1471-1484.
8.X. Jia, H. Zeng, W. Wang, F. Zhang, and H. Yin, Chitosan oligosaccharide induces resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana by activating both salicylic acid- and jasmonic acid-mediated pathways. Molecular Plant-Microbe Interactions 31 (2018) 1271-1279.
9.J. Zheng, X. Yuan, C. Zhang, P. Jia, S. Jiao, X. Zhao, H. Yin, Y. Du, and H. Liu, N-Acetylcysteine alleviates gut dysbiosis and glucose metabolic disorder in high-fat diet-fed mice. Journal of Diabetes (2018).
10.H. Cao, M. Yue, G. Liu, Y. Du, and H. Yin, Microbial production of mannitol by Lactobacillus brevis 3-A5 from concentrated extract of Jerusalem artichoke tubers. Biotechnology and Applied Biochemistry 65 (2018) 484-489.
11.K. Li, W. Chen, W. Wang, H. Tan, S. Li, and H. Yin, Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase. Carbohydrate Polymers 201 (2018) 122-130.
12.Y. He, S.K. Bose, W. Wang, X. Jia, H. Lu, and H. Yin, Pre-Harvest Treatment of Chitosan Oligosaccharides Improved Strawberry Fruit Quality. International Journal of Molecular Sciences 19 (2018) 2194.
13.H. Tan, W. Chen, Q. Liu, G. Yang, and K. Li, Pectin Oligosaccharides Ameliorate Colon Cancer by Regulating Oxidative Stress-and Inflammation-Activated Signaling Pathways. Frontiers in Immunology 9 (2018).
14. J. He, R. Li, X. Sun, W. Wang, J. Hu, H. Xie, and H. Yin, Effects of Calcium Alginate Submicroparticles on Seed Germination and Seedling Growth of Wheat (Triticum aestivum L.). Polymers 10 (2018) 1154.
15.Y. He, S.K. Bose, M. Wang, T. Liu, W. Wang, H. Lu, and H. Yin, Effects of chitosan oligosaccharides postharvest treatment on the quality and ripening related gene of cultivated strawberry fruits. Journal of Berry Research (2018) DOI: 10.3233/JBR-180307.
16.Q. Xu, W. Wang, C. Qu, J. Gu, H. Yin,Z. Jia, L. Song, and Y. Du, Chitosan oligosaccharides inhibit epithelial cellmigration through blockade of N-acetylglucosaminyltransferase V and branchedGlcNAc structure.CARBOHYDRATE POLYMERS 170 (2017) 241-246.
17.H. Cao, M. Yue, G. Liu, Y. Du, and H.Yin, Microbial production of mannitol by Lactobacillus brevis 3-A5 fromconcentrated extract of Jerusalem artichoke tubers.BIOTECHNOLOGY AND APPLIEDBIOCHEMISTRY(2017).
18.W. Lei, D. Wang, T. Dou, J. Hou, L.Feng, H. Yin, Q. Luo, J. Sun, G. Ge, and L. Yang, Assessment of the inhibitoryeffects of pyrethroids against human carboxylesterases.TOXICOLOGY AND APPLIEDPHARMACOLOGY 321 (2017) 48-56.
19.Q. Xu, M. Liu, Q. Liu, W. Wang, Y. Du,and H. Yin, The inhibition of LPS-induced inflammation in RAW264.7 macrophagesvia the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide.CARBOHYDRATE POLYMERS 174 (2017) 1138-1143.
20.R. Jia, K. Sun, R. Li, Y. Zhang, W.Wang, H. Yin, D. Fang, Q. Shi, and Z. Tan, Heat capacities of some sugaralcohols as phase change materials for thermal energy storage applications.JOURNAL OF CHEMICAL THERMODYNAMICS 115 (2017) 233-248.
21.H. Zheng, M. Gao, Y. Ren, R. Lou, H.Xie, W. Yu, X. Liu, and X. Ma, An improved pH-responsive carrier based onEDTA-Ca-alginate for oral delivery of Lactobacillus rhamnosus ATCC 53103.CARBOHYDRATE POLYMERS 155 (2017) 329-335.
22. Xiaochen Jia, Qingshan Meng, Haihong Zeng, Wenxia Wang, Heng Yin, Chitosan oligosaccharide induces resistance to Tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway; Scientific Reports, 2016, 6: 26144
23. Heng Yin, Yuguang Du, Zhongmin Dong. Chitin Oligosaccharide and Chitosan Oligosaccharide: Two Similar but Different Plant Elicitors. Front Plant Sci. 2016, 7: 522.
24. Benwei Zhu, Meijuan Chen, Heng Yin, Yuguang Du, Limin Ning, Enzymatic hydrolysis of alginate to produce oligosaccharides by a new purified endo-type alginate lyase； Mar. Drugs. 2016, 14(6): 108
25. Likun Cheng, Qingsong Xu, Kaiyun Yang, Jun He, Dawen Chen, Yuguang Du, Heng Yin, Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with Q-TOF tandem mass spectrometry. Electrophoresis. 2016, 37: 1525-1531.
26. Xiaochen Jia, jian Kang, Heng Yin, A simple and rapid method for measuring alpha-D-phosphohexomutases activity by using anion-exchange chromatography coupled with an electrochemical detector. PeerJ. 2016, 4: e1517.
27. Junyan Ma, Hailong Cao, Haidong Tan, Xuejun Hu, Wujun Liu, Yuguang Du, Heng Yin, Cloning, Expression, Characterization, and Mutagenesis of a Thermostable Exoinulinase From Kluyveromyces cicerisporus. Appl Biochem Biotechnol. 2016, 178(1): 144-158.
28. Xiaohui Wang, Naiyu Chi, Fengwu Bai, Yuguang Du, Yong Zhao, Heng Yin, Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp. DL-6. Extremophiles. 2016, 20(2): 167-176.
29. Huiyan Zhang, Yong Zhao, Hailong Cao, Guangqing Mou, Heng Yin, Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis, International Journal of Biological Macromolecules, 2015, 79: 72–75.
30. Benwei Zhu, Heng Yin, Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications, Bioengineered, 2015, 6(3): 125-131 (Cover story).
31. Benwei Zhu, Haidong Tan，Yuqi Qin，Qingsong Xu，Yuguang Du，Heng Yin，Characterization of a newendo-type alginate lyase from Vibrio sp. W13，International Journal of Biological Macromolecules，2015，75: 330-337
32. Benwei Zhu，Lishuxin Huang，Haidong Tan，Yuqi Qin，Yuguang Du，Heng Yin，Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp.，Biotechnology Letters，2015，37: 409-415
33. Mengyu Wang, Yinchao Chen, Rui Zhang, Wenxia Wang, Xiaoming Zhao, Yuguang Du, Heng Yin，Effects of chitosan oligosaccharides on the yield components andproduction quality of different wheat cultivars (Triticum aestivum L.) in Northwest China, Field Crops Research，2015，172: 11–20
34. LK Cheng, LX Wang, QS Xu, LJ Huang, DS Zhou, Z. Li, SG Li, YG Du, Heng Yin，Chitooligosaccharide supplementation improves the reproductive performance and milk composition of sows, Livestock Science, 2015, 174: 74-81.
35. Xiaohui Wang, Yong Zhao, Haidong Tan, Naiyu Chi, Qingfang Zhang, Yuguang Du, Heng Yin, Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium, , International Journal of Biological Macromolecules, 2014, 70: 455–462.
36. Yu Li, Qiongsong Xu, Peng Wei, Likun Cheng, Qiang Peng,Shuguang Li, Heng Yin, Yuguang Du. Chitosan oligosaccharides negatively regulate MAP kinase signaling to ablate the expressions of E-selectin and ICAM-1 induced by LPS in endothelial cells. International Journal of Molecular Medicine. 2014, 33(2): 392-400.
37. Yunhong Zhang, Heng Yin, Xiaoming Zhao, Wenxia Wang, Yuguang Du, Ailing He, Kegang Sun, The promoting effects of alginate oligosaccharides on root development in Oryza sativa L. mediated by auxin signaling. Carbohydrate Polymers, 2014, 113: 446-454.
38. Qiang Peng, Qingsong Xu, Heng Yin, Linjuan Huang, Yuguang Du, Characterization of an immunologically active pectin from the fruits of Lycium ruthenicum. International Journal of Biological Macromolecules, 2014, 64: 69–75.
39. Heng Yin, Yan Li, Hongyan Zhang, WenXia Wang, Hang Lu, Kai Grevsen, Xiaoming Zhao, Yuguang Du, Chitosan oligosaccharides-triggered innate immunity contributes to oilseed rape resistance against Sclerotinia Sclerotiorum, International Journal of Plant Sciences, 2013, 174(4): 722-732 (Cover story).
40. Yunhong Zhang, Heng Yin, Wang WX, Zhao XM, Du YG, Wu LS. Enhancement in photosynthesis characteristics and phytohormones of flowering Chinese cabbage (Brassica campestris L. var. utilis Tsen et Lee) by exogenous alginate oligosaccharides. Journal of Food, Agriculture & Environment. 2013, 11(1): 669-675.
41. Yunhong Zhang, Heng Yin, Liu H, Wang WX, Wu LS, Zhao XM, Du YG. Alginate oligosaccharides regulate nitrogen metabolism via calcium in Brassica campestris L. var. utilis Tsen et Lee. Journal of horticultural Science and Biotechnology, 2013, 88(4): 502-508.
42. Hang Liu, Yunhong Zhang, Heng Yin, Wenxia Wang, Xiaoming Zhao, Yuguang Du, Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress, Plant Physiology and Biochemistry, 2013, 62: 33-40.
43. Yunhong Zhang, Hang Liu, Heng Yin, Wang WX, Zhao XM, Du YG. Nitric oxide mediates alginate oligosaccharides induced root development in Triticum aestivum L. Plant Physiology and Biochemistry, 2013, 71: 49-56.
44. Heng Yin, XC Frette, LP Christensen, Kai Grevsen, Chitosan oligosaccharides promote the content of polyphenols in Greek Oregano (Origanum vulgare ssp hirtum), Journal of Agriculture and Food Chemistry, 2012, 60(1): 136-143.
45. Heng Yin*, Anders Kjaer, Xavier C. Fretté, Yuguang Du, Lars P. Christensen, Martin Jensen, Kai Grevsen, Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L, Process Biochemistry, 2012, 47: 1559-1562.
46. Weihua Guo, Heng Yin, Zhiqiang Ye, Xiaoming Zhao, Jingli Yuan, Yuguang Du, A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method, Carbohydrate Polymers, 2012, 90(1): 491-495.
47. Hongyan Zhang, Wenxia Wang, Heng Yin, Xiaoming Zhao, Yuguang Du, Oligochitosan induces programmed cell death in tobacco suspension cells, Carbohydrate Polymers, 2012, 87(3): 2270-2278.
48. Yunbin Fu, Heng Yin, Wenxia Wang, Mengyu Wang, Hongyan Zhang, Xiaoming Zhao, Yuguang Du, beta-1,3-Glucan with different degree of polymerization induced different defense responses in tobacco, Carbohydrate Polymers, 86, 2, 774-782, 2011
49. Hongyan Zhang, Xiaoming Zhao, Jinli Yang, Heng Yin, Wenxia Wang, Hang Lu, Yuguang Du, Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide, Plant Cell Reports, 30, 6, 1153-1162, 2011
50. Heng Yin, Xiaoming Zhao, Yuguang Du, Oligochitosan: A plant diseases vaccine, Carbohydrate Polymers, 82, 1-8, 2010
51. Heng Yin, Xiaoming Zhao, Xuefang Bai, Yuguang Du, Molecular cloning and characterization of a Brassica napus L. MAP Kinase involved in oligochitosan-induced defense signaling, Plant Molecular Biology Report, 28, 292–301, 2010
52. Hang Lu, Xiaoming Zhao, Wenxia Wang, Heng Yin, Junguang Xu, Xuefang Bai, Yuguang Du, Inhibition effect on tobacco mosaic virus and regulation effect on calreticulin of oligochitosan in tobacco by induced Ca2+ influx, Carbohydrate Polymers, 82, 1, 136-142, 2010
53.何金霞, 贾晓晨, 王文霞, 胡建恩, 尹恒. 植物Ｎ-糖链检测技术研究进展. 生物技术进展 8 (2018) 500-508.
54.贾晓晨,尹恒. 植物蛋白O-GlcNAc糖基化及生物学功能. 生物化学与生物物理进展(2017) 919-928.
55. 尹恒，赵小明，王文霞，杜昱光. 植物糖生物学与糖链植物疫苗(约稿), 生命科学, 第23卷, 第6期, 598-604, 2011
56. 尹恒, 王文霞, 赵小明, 杜昱光. 植物糖生物学研究进展(特邀综述), 植物学报, 第45卷, 第5期, 521–529, 2010
57. 王文霞，赵小明，杜昱光，尹恒. 寡糖生物防治应用及机理研究进展（约稿）. 中国生物防治学报. 2015(5): 757-769.
58. 吴树丽，刘启顺，谭海东，张付云，尹恒. 5- 羟甲基糠醛的生物催化氧化研究进展. 生物技术通报. 2016, 32(9): 50-58.
专著
1. Heng Yin, Yuguang Du,《Research Progress in Oligosaccharins》, Chief Editor, Springer-Verlag Press, ISBN 978-1-4939-3516-1. 2016
2. Heng Yin, Yuguang Du. Chapter 41: Mechanism and Application of Chitin/Chitosan and Their Derivatives in Plant Protection. in Chitin, Chitosan, Oligosaccharides and Their Derivatives: Biological Activities and Applications, 605-617. Editor: Se-Kwon Kim Publisher: CRC Press, Taylor & Francis Group. ISBN: 978-1-4398-1603-5. 2010
3. 尹恒，杜昱光，第一章：植物免疫系统与糖链植物疫苗，《糖链植物疫苗研究与应用》，主编：杜昱光，赵小明，尹恒，王文霞，科学出版社，28.8万字，2012
4.尹恒，傅赟彬，张洪艳，王文霞，杜昱光，第三章：糖链植物疫苗作用机理研究进展，《糖链植物疫苗研究与应用》，主编：杜昱光，赵小明，尹恒，王文霞，科学出版社，28.8万字，2012
5. 尹恒，王文霞，杜昱光，第五章：植物糖生物学与糖链植物疫苗，《糖生物工程》，主编：张树政，金城，杜昱光，化学工业出版社，46.7万字，2012
国际会议报告
1.Jun 2018 Kyoto Japan, The 15th Japan-China-Korea Joint Symposium on Enzyme, Oral report.
2.Jun 2018 Qingdao China, The 11th International Symposium on Glycosyltransferases , Oral report.
3.May 2018 Beijing China, The First International Congress of Biological Control, Oral report.
4.May 2018 Boao China, The International Conference on Green Plant Protection Innovation, Oral report.
5. Nov 2016 Nanning China, The 14th China-Japan-Korean Joint Symposium on Enzyme Engineering, Oral report.
6. Sep 2015 Split Croatia, 23rd International Symposium on Glycoconjugates, Oral report.
7. August 2015 Qingdao China, 3rd International Conference on Coastal Biotechnology, Oral report.
8. Noverber 2014 Jeju Korea, The 13th China-Japan-Korean Joint Symposium on Enzyme Engineering, Oral report.
9. August 2013 Beijing China, 2013 10th International Congress of Plant Pathology, Oral report.
10. July 2013 Shanghai China, 2013 International Symposium on Chemical Glycobiology, Oral report.
11. June 2013 Dalian China, 22nd International Symposium on Glycoconjugates, Oral report.
12. May 2011 Shanghai China, Asian Congress on Biotechnology, Oral report.
13. April 2010 Saitama Japan, Second Japan/China Joint Symposium on Glycobiology, Oral report.
科研项目
主持的主要项目
1. 国家自然科学基金面上项目“含CBM1结构域的多糖裂解单加氧酶鉴定及其底物结合机制研究”，2017.1-2020.12；
2.国家重点研发计划课题“功能糖类免疫调控技术及产品开发”, 2017.07-2020.12;
3.中国科学院科技服务网络计划“ 集成与示范绿色生物防控减肥减药技术”, 2018.08-2019.12;
4. 国家自然科学基金面上项目“拟南芥磷酸N-乙酰氨基葡萄糖变位酶功能鉴定及其影响蛋白糖基化的研究”，2014.1-2017.12；
5. 国家科技支撑计划课题“海藻寡糖饲料添加剂的制备及质量控制研究”，2013.1-2016.12；
6. 国家自然科学青年基金“烟草壳寡糖受体鉴定及其在诱抗过程中的功能确认”，2011.1-2013.12；
7. 中国科学院STS课题“新型糖类与微生物农药及抗逆剂创制及示范”，2016.1-2017.12；
8. 大连市杰出青年科技人才“新型海洋寡糖农用制剂研发及应用推广”，2016.1-2018.12；
9. 辽宁省自然科学基金“稻瘟病菌中多糖单加氧酶鉴定及功能解析”，2015.1-2017.12；
10. 辽宁省博士启动基金“褐藻胶裂解酶基因工程改造及褐藻胶寡糖制备技术研究”，2011.1-2013.12；
参与的主要项目
1. 国家863计划“新型海洋生物农用制剂产品开发”，2011.7-2014.6；
2. 国家863计划“海洋生物农药及植物促生长剂产业化关键技术研究”，2007.7-2010.6；
3. 公益性行业（农业）科研专项“新型寡糖植物疫苗的研制与产业化开发”，2009.1-2013.12。
兴趣爱好
1.足球：既看且踢。罗马、瓦伦西亚和本地俱乐部球迷。
2.阅读：偏好历史、科幻与推理小说，属意作家为田中芳树。